Hybrid glycosaminoglycans synthesized by monolayers of chick embryo arterial fibroblasts

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Abstract
Monolayer cultures of arterial fibroblasts from 13-day chick embryonic aorta incorporated 35SO42- into glycosaminoglycans containing glucuronic and iduronic acids. Bacterial chondroitinase ABC converted more than 98% of the 35SO4-labeled polymer to mono- or disaccharides, including N-acetyl-D-galactosamine 4-sulfate, .DELTA.4,5-glucuronic acid 2- or 3-sulfate .fwdarw. N-acetylgalactosamine 6-sulfate and the unsaturated disaccharides normally obtained from chondroitin 4-sulfate and chondroitin 6-sulfate sequences. Chondroitinase AC converted only 77% of the 35SO4-labeled polymer to the same mono- and disaccharides and also yielded the following oligosaccharide products: .DELTA.4,5-glucuronic acid .fwdarw. N-acetylgalactosamine 4- or 6-sulfate .fwdarw. iduronic acid .fwdarw. N-acetylgalactosamine 6- or 4-sulfate; N-acetylgalactosamine 4-sulfate .fwdarw. iduronic acid 2- or 3-sulfate .fwdarw. N-acetylgalactosamine 6-sulfate; .DELTA.4,5-glucuronic acid .fwdarw. N-acetylgalactosamine 4-sulfate .fwdarw. (iduronic acid .fwdarw. N-acetylgalactosamine 4-sulfate)2; .DELTA.4,5-glucuronic acid .fwdarw. N-acetylgalactosamine 4- or 6-sulfate .fwdarw. (iduronic acid .fwdarw. N-acetylgalactosamine 6- or 4-sulfate)2; higher oligosaccharides containing iduronic acid and N-acetylgalactosamine 4-sulfate.