Deoxyribonucleic Acid Base Sequence Studies on Glucan-Producing and Glucan-Negative Strains of Streptococcus mitior

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Abstract
The DNA base sequence similarities among 14 strains of S. mitior were assessed by hybridization. Some strains produced glucan. DNA was purified by a conventional method and by centrifugation in CsCl. Hybridizations were done in solution with S1 nuclease and on membrane filters. The results showed 2 groups of moderately homologous strains. The glucan-producing strains represented a group which included some glucan-negative strains. Hybridization by the S1 method was less than with the filter method, indicating some sequence divergence within the 2 groups. There was a slight difference in guanine-plus-cytosine contents of the 2 groups.