Isolation of the Corynebacterium glutamicum glnA gene encoding glutamine synthetase I
Open Access
- 1 September 1997
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 154 (1) , 81-88
- https://doi.org/10.1016/s0378-1097(97)00304-2
Abstract
The Corynebacterium glutamicum glutamine synthetase I (GSI) structural gene glnA was cloned by a PCR approach using oligonucleotide primers derived from conserved amino acid sequences of the GSI proteins from various bacteria. Disruption or deletion of this gene in C. glutamicum led to a glutamine auxotrophic phenotype and complete loss of glutamine synthetase activity, indicating the key role of this enzyme in nitrogen metabolism. Additionally, indications for a second glutamine synthetase, GSII, were found.Keywords
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