Sequence analysis and transcriptional profiling of two vacuolar H+-pyrophosphatase isoforms in Vitis vinifera
- 19 August 2006
- journal article
- Published by Springer Nature in Journal of Plant Research
- Vol. 119 (5) , 469-478
- https://doi.org/10.1007/s10265-006-0009-4
Abstract
Gene expression of grapevine vacuolar H+-pyrophosphatase (V-PPase EC 3.6.1.1.) during fruit ripening has previously been reported. Here we report on putative multiple V-PPase isoforms in grapevine. In this study a full-length cDNA sequence with an open reading frame of 2,295 nucleotides encoding a V-PPase gene (vpp2: acc. nr. AJ557256) was cloned. Sequence analyses of the deduced amino acid residues and RT-PCR experiments indicated that Vitis vinifera L. has at least two distinct isoforms of the V-PPase gene. Bioinformatic analyses of 13 V-PPase protein sequences revealed two highly conserved motifs associated with pyrophosphate (PPi) binding and response to stress, respectively. Both V-PPase isoforms were expressed at higher levels in the late post-véraison stage of grape berry ripening. Results also showed that the expression of grapevine V-PPase was induced by cold stress.Keywords
This publication has 40 references indexed in Scilit:
- Kinetics of pyrophosphate-driven proton uptake by acidocalcisomes of Leptomonas wallaceiBiochemical and Biophysical Research Communications, 2005
- Membrane Topology of the H+-pyrophosphatase of Streptomyces coelicolor Determined by Cysteine-scanning MutagenesisPublished by Elsevier ,2004
- Effect of salt and osmotic stresses on the expression of genes for the vacuolar H+-pyrophosphatase, H+-ATPase subunit A, and Na+/H+ antiporter from barleyJournal of Experimental Botany, 2004
- 14‐3‐3 proteins: regulation of signal‐induced eventsPhysiologia Plantarum, 2004
- Mutagenic Analysis of Functional Residues in Putative Substrate-binding Site and Acidic Domains of Vacuolar H+-PyrophosphatasePublished by Elsevier ,2001
- Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. CohenJournal of Molecular Biology, 2001
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Properties of proton and sugar transport at the tonoplast of tomato (Lycopersicon esculentum) fruitPhysiologia Plantarum, 1995
- Vacuolar H+-Translocating Pyrophosphatase Is Induced by Anoxia or Chilling in Seedlings of RicePlant Physiology, 1995
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994