Linear Photodiode Array Spectrometers As Detector Systems in Automated Liquid Chromatographs

Abstract
The development of two rapid scanning spectrometer systems for use as liquid chromatographic detector systems will be discussed. The first involves a minimum configuration involving a microprocessor and a linear photodiode array acting as a rapid scanning single beam UV/VIS detector. The instrument can monitor up to eight wave single-wave lengths. It plots the absorption at the selected wave lengths as a function of time on a standard X/Y plotter. It can take a complete spectrum about every five seconds — the time being limited mainly by the low slow speed of the recorder. The second system was designed around a large minicomputer system and is intended for pattern recognition work focusing on metabolite characterization, isolation, and identification. It is capable of taking 20 spectra/second in a dual-beam mode. Examples of the systems in practical applications will be shown, and a detailed analysis of the instruments design features will be presented.