CHROMOSOMAL LOCALIZATION OF THE HUMAN PLACENTAL LACTOGEN-GROWTH HORMONE GENE-CLUSTER TO 17Q22-24
- 1 January 1982
- journal article
- research article
- Vol. 34 (2) , 227-234
Abstract
Recombinant plasmid HCS-pBR322 containing a 550-base-pair (bp) insert of c[complementary]DNA to human placental lactogen (hPL) mRNA was 3H-labeled by nick translation and hybridized in situ to human chromosome preparations in the presence of 10% dextran sulfate. A high percentage of cells (80%) were found to exhibit label on the distal end of the long arm of chromosome 17. Ag grains on this region constituted 25.5% of all labeled sites, allowing assignment of the hPL and growth hormone (hGH) genes, which have over 90% nucleotide homology in their coding sequences, to 17q22-24. A gene copy number experimental showed that both genes are present in .apprx. 3 copies/haploid genome.This publication has 24 references indexed in Scilit:
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