Cloning of the structural gene for orotidine 5′-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli
- 1 June 1983
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 190 (3) , 403-405
- https://doi.org/10.1007/bf00331067
Abstract
A Neurospora gene bank in plasmid pRK9 was used to complement pyrimidine auxotrophs in E. coli. Two plasmids were obtained that complement a pyrF mutant of E. coli. These plasmids hybridise to Neurospora DNA and transform a pyr-4 strain of Neurospora. The promoter used in expressing the orotidine 5′-monophosphate carboxylase in E. coli is within the Neurospora sequence.This publication has 12 references indexed in Scilit:
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