A colorimetric method for the estimation of tyrosine

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Abstract
A simple and reliable method of estimating tyrosine was developed which depends on the reaction between tyrosine and 1-nitroso-2-naphthol in the presence of an oxidizing agent (HNO3). A reagent containing NaCl and FeCl3 is added after color development to stabilize the color. The composition of the stabilizing agent and the volume to be added may be altered as convenient so long as the final solution contains 0.12 [image] NaCl and 25% acetone. The method is suitable for the analysis of 10-250 ug of tyrosine in 2-4 ml samples. The effects of a number of interfering substances were investigated and precautions are described for the determination of tyrosine in various biological fluids.