A Quantitative Immunocytochemical Study of the Luteinizing Hormone and Follicle-Stimulating Hormone Cells in the Adenohypophysis of Adult Male Rats and Adult Female Rats throughout the Estrous Cycle*

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Abstract
It was investigated whether the absolute or the relative numbers of LH [luteinizing hormone] and FSH cells change during the rat estrous cycle, whether the percentages of gonadotrophs that contain LH and/or FSH change during the estrous cycle and whether gonadotrophs change in size during the rat estrous cycle. Groups of 4 female rats were decapitated at 1 of 5 different times during the estrous cycle. Four male rats were also decapitated. Serum concentrations of LH and FSH were determined by RIA [radioimmunoassay]. Paired horizontal flip-flopped or nonflipped paraffin sections were mounted from the dorsal, middle and ventral portions of each pituitary gland. In each pair of sections, one was stained with a-rat LH-S4 and the other with a-rat FSH-S7 by the unlabeled antibody peroxidase-antiperoxidase method. All immunoreactive cells were counted. Photographs were taken from randomly chosen corresponding areas, and the cells were individually matched to determine the percentage that contained 1 or both hormones. Correction factors had to be used because in paired flip-flopped or nonflipped sections stained with the same antibody (a-rat LH-S4), not all of the stained cells found in 1 section were found in the other section. The absolute numbers of LH and FSH cells did not change throughout the estrous cycle. The ratio of LH cells to FSH cells in the pars distalis of female rats was also constant throughout the estrous cycle. In females rats, 75.2% of LH cells also contained FSH, while 99.4% of FSH cells also contained LH. In the male rats, 86.6% of LH cells also contained FSH, while 98.6% of FSH cells also contained LH. Similar results were obtained in paired flip-flopped sections stained with a-rat LH.beta. and a-rat FSH.beta.. Sequential staining of additional individual tissue sections with a-rat LH-S4 and then a-rat FHS-S7 or vice versa revealed the following. Staining of LH-stained tissue for FSH revealed less than 1% new cells, but staining of FSH-stained tissue for LH revealed a 8.7% increase in gonadotrophs in males and a 25.4% increase in females. The gonadotrophs in female rats did not change in size during the estrous cycle and were significantly smaller than the gonadotrophs in male rats. Apparently in normal adult rats virtually all FSH-containing cells contain LH, about 25% of the gonadotrophs in males contain LH but not FSH, the number of cells containing LH or those containing LH and FSH does not change during the estrous cycle, gonadotrophs in female rats do not change in size during the estrous cycle and are smaller than the gonadotrophs in male rats, and FSH release during the early morning of estrus, when the serum FSH concentration is elevated and the serum LH concentration is low, occurs from cells that contain both LH and FSH.

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