Human Immunodeficiency Virus Bearing a Disrupted Central DNA Flap Is Pathogenic In Vivo
- 1 June 2007
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 81 (11) , 6146-6150
- https://doi.org/10.1128/jvi.00203-07
Abstract
The central DNA flap is an important component of lentiviral vectors, but its significance in the context of wild-type human immunodeficiency virus (HIV) is currently unclear. To address this issue, we have compared the in vitro infection kinetics of NL4-3 with those of a flap-deficient mutant and evaluated the in vivo growth characteristics of these viruses by using the SCID-hu mouse model of HIV infection. Flap-deficient virus was only modestly attenuated in vitro, as assessed by single-round and spreading infection assays, and exhibited levels of replication and pathogenesis close to those of the wild-type in vivo. Hence, an intact central flap is not essential for HIV replication.Keywords
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