Polysaccharides in Ulcerative Colitis and Normal Human Rectum Compared by Stains of Biopsy Specimens

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Abstract
The lysozyme substrate contained in Micrococcus lysodeikticus is stained by the Hotchkiss or McManus technic after fixation and sectioning by the same procedure utilized for tissue. After fixation and sectioning, the substrate remains susceptible to lysis by lysozyme. Egg albumin, as used in application of sections to slides, does not lyse the lysozyme substrate. Biopsy specimens of rectal mucosa from normal persons and patients with active ulcerative colitis after various methods of fixation show no consistent difference in reaction to the Hotchkiss or McManus stain. In vitro application of lysozyme and hyaluron-idase to human rectal tissue of normal individuals and patients with ulcerative colitis does not alter the reaction of the tissue to these stains.