Cyclic nucleotide content and protein phosphorylation during maturation ofSpisula oocytes

1 March 1987

journal article
research article
Published by Wiley in Gamete Research

Vol. 16 (3) , 251-258
https://doi.org/10.1002/mrd.1120160307

Abstract

Cyclic nucleotide levels in the oocytes of the surf clam Spisula solidissima were measured during germinal vesicle breakdown (GVBD) induced by fertilization. The level of cAMP and cGMP in untreated oocytes was 8.23 ± 0.95 and 4.89 ± 0.39 pmol/10⁶ oocytes. The ratio of cAMP to cGMP ranged from 1.5 to 2.0. The cAMP level in Spisula oocytes fluctuated after fertilization and before GVBD. The cGMP level showed minimal fluctuation, with a tendency to decrease initially followed by a subsequent rise to the basal level in a nonsynchronous manner. These changes were not statistically significant. There was a general increase in protein phosphorylation during the period after fertilization and before GVBD. The greatest increase occurred with proteins of estimated molecular weights of 52, 18, and 12 kD, analyzed by gel electrophoresis and autoradiography.

Keywords

This publication has 30 references indexed in Scilit:

Progesterone and cAMP-dependent protein kinase regulate in vivo the level of phosphorylation of two proteins (Mr 20,000 and Mr 32,000) in Xenopus oocytes
Developmental Biology, 1986
Induction of early mitotic events in a cell-free system
Cell, 1985
Stimulation of protein phosphorylation during fertilization-induced maturation of Urechis caupo oocytes
Developmental Biology, 1982
Fertilization of sea urchin eggs is accompanied by 40 S ribosomal subunit phosphorylation
Developmental Biology, 1981
Insulin‐like growth factor: insulin or serum increase phosphorylation of ribosomal protein S6 during transition of stationary chick embryo fibroblasts into early G₁ phase of the cell cycle
FEBS Letters, 1979
Epidermal growth factor stimulates phosphorylation in membrane preparations in vitro
Nature, 1978
Changes in protein phosphorylation accompanying maturation of Xenopus laevis oocytes
Developmental Biology, 1977
Protein phosphorylation during oocyte maturation
Nature, 1974
Role for Protein Phosphorylation in Meiosis and in the Early Cleavage Phase of Amphibian Embryonic Development
Nature, 1972
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Nature, 1970