RNA-reverse transcriptase complex from cultured human myeloma-leukemia cells
- 14 July 1977
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 20 (1) , 15-20
- https://doi.org/10.1002/ijc.2910200105
Abstract
A high molecular weight RNA‐reverse transcriptase complex in the culture media of peripheral leukocytes obtained from two Japanese patients with myeloma‐leukemia was detected by demonstration of a 3H‐uridine peak and a peak of DNA polymerizing activity banding at a density of 1.15–1.19 g/ml. The enzyme in the complex was able to utilize poly(rA) · d(pT)10 or poly (rC) · d(pG)12–18, but not poly (dA) · d(pT)10 or (dT)12–18, as template‐primers. The sucrose density sedimentation analysis revealed that RNA in the complex sedimented at a location of approximately 50s and 20–30s.This publication has 17 references indexed in Scilit:
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