Studies on the particulate components of rat mammary gland. 1. A method for determining the composition of the retained fluid

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Abstract
The levels of DNA, lactose and total N in suspensions of rat mammary-gland tissue were determined at intervals during the lactation cycle. A method is described by which the lactose and N levels of colostrum and milk at periods close to parturition can be determined. Values were calculated for the contribution by tissue and retained fluid to the total N of the suspensions. The tissue N increases slowly during pregnancy, very rapidly over the period of parturition and then slowly again during lactation.