Molecular Cloning and Expression of Mouse Placental Lactogen I Complementary Deoxyribonucleic Acid
- 1 November 1987
- journal article
- research article
- Published by The Endocrine Society in Molecular Endocrinology
- Vol. 1 (11) , 767-776
- https://doi.org/10.1210/mend-1-11-767
Abstract
The mouse midpregnancy lactogen or placental lactogen I (mPL-I) is encoded by a 1.0-kilobase mRNA that appears transiently during gestation, with maximal amounts accumulating in the placenta at day 10 of pregnancy. Several cDNA clones for mPL-I have been isolated from a .lambda.gt11 expression library constructed from day 10-placental RNA. The cDNA sequence indicates that mPL-I is synthesized as a 224 amino acid precursor, and is secreted as a 194 amino acid glycosylated hormone. The deduced amino acid sequence of mPL-I is highly homologous to the known members of the PRL family in the mouse, and hybridization analysis indicates that the mouse genome contains several mPL-I genes. Introduction of the mPL-I cDNA in an expression vector into cultured mouse cells results in the synthesis and secretion of glycosylated mPL-I protein that is recognized by anti-mPL-I antiserum and is biologically active.Keywords
This publication has 29 references indexed in Scilit:
- TWO FORMS OF RAT PLACENTAL LACTOGEN REVEALED BY RADIOIMMUNOASSAYEndocrinology, 1981
- A New Sensitive and Specific Bioassay for Lactogenic Hormones: Measurement of Prolactin and Growth Hormone in Human Serum*Journal of Clinical Endocrinology & Metabolism, 1980
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- Early events in the biosynthesis of the lysosomal enzyme cathepsin D.Journal of Biological Chemistry, 1979
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- The Pattern of Plasma Prolactin Concentration during the First Half of Mouse Gestation1Biology of Reproduction, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexaminationBiochemistry, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976