DOES RESTRICTION ENZYME ANALYSIS REVEAL CHANGES IN HERPES-SIMPLEX VIRUS-DNA AFTER LATENCY IN MOUSE GANGLIA

  • 1 April 1986
    • journal article
    • research article
    • Vol. 9  (2) , 139-145
Abstract
Possible changes in the herpes simplex virus (HSV) genome after latent infection in mice were studied by restriction enzyme analysis. Lumbosacral ganglia of C57Bl mice, genitally infected with a low passage strain of HSV-1 were explanted 3-8 month postinfection and cocultivated with human embryo fibroblasts. Four virus isolates reactivated from the ganglia were compared with the original virus inoculated into the mice. The cleavage patterns yielded by 7 restriction enzymes - i.e. Eco RI, Hind III, Bam HI, Kpn I, Xba I, Bg1 II, HpaI-did not indicate any differences.