Differentiation of Amino Acids by Gas-Liquid Chromatography of their Pyrolysis Products

Abstract
Conditions are described for the low-temperature pyrolysis of amino acids and gas-liquid chromatography of the amines produced. Pyrolysis is accomplished at 300°C, and the amines are stabilized at 110°C prior to chromatography on Quadrol. Aliphatic amines C1 to C5 can be observed under these conditions. Each amino acid gives a unique amine profile, and proteins give reproducible amine profiles related to their amino acid content.

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