Laser-capture microdissection
Top Cited Papers
- 27 June 2006
- journal article
- Published by Springer Nature in Nature Protocols
- Vol. 1 (2) , 586-603
- https://doi.org/10.1038/nprot.2006.85
Abstract
Deciphering the cellular and molecular interactions that drive disease within the tissue microenvironment holds promise for discovering drug targets of the future. In order to recapitulate the in vivo interactions thorough molecular analysis, one must be able to analyze specific cell populations within the context of their heterogeneous tissue microecology. Laser-capture microdissection (LCM) is a method to procure subpopulations of tissue cells under direct microscopic visualization. LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. A variety of downstream applications exist: DNA genotyping and loss-of-heterozygosity (LOH) analysis, RNA transcript profiling, cDNA library generation, proteomics discovery and signal-pathway profiling. Herein we provide a thorough description of LCM techniques, with an emphasis on tips and troubleshooting advice derived from LCM users. The total time required to carry out this protocol is typically 1-1.5 h.Keywords
This publication has 82 references indexed in Scilit:
- Detection and identification of heat shock protein 10 as a biomarker in colorectal cancer by protein profilingProteomics, 2006
- Significant Angiogenic Potential is Present in the Microenvironment of Muscle Flaps in HumansJournal of Reconstructive Microsurgery, 2005
- Differential Radioactive Proteomic Analysis of Microdissected Renal Cell Carcinoma Tissue by 54 cm Isoelectric Focusing in Serial Immobilized pH Gradient GelsJournal of Proteome Research, 2005
- Proteomic Analysis of Apoptotic Pathways Reveals Prognostic Factors in Follicular LymphomaClinical Cancer Research, 2005
- Mapping Molecular Networks Using Proteomics: A Vision for Patient-Tailored Combination TherapyJournal of Clinical Oncology, 2005
- Reversing the Effects of Formalin Fixation with Citraconic Anhydride and Heat: A Universal Antigen Retrieval MethodJournal of Histochemistry & Cytochemistry, 2005
- Signal pathway profiling of ovarian cancer from human tissue specimens using reverse‐phase protein microarraysProteomics, 2003
- Signal pathway profiling of prostate cancer using reverse phase protein arraysProteomics, 2003
- Navigated laser capture microdissection as an alternative to direct histological staining for proteomic analysis of brain samplesProteomics, 2003
- Cut out or poke in—the key to the world of single genes: laser micromanipulation as a valuable tool on the look-out for the origin of diseaseGenetic Analysis: Biomolecular Engineering, 1997