Metabolic activation of 3-methylcholanthrene in mouse skin: fluorescence spectral evidence indicates the involvement of diol-epoxides formed in the 7, 8, 9, 10-ring

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Abstract
Hydrolysates of DNA that had been isolated from mouse skin treated with 3H-labelled 3-methylcholanthrene were subjected to chromatography on Sephadex LH20 columns and 3H-labelled products that eluted in the region expected for nucleoside-hydrocarbon adducts were purified further by high pressure liquid chromatography; eight major and one minor products were resolved and their fluorescence spectra were determined using photon-counting spectrophotofluorimetry. The fluorescence spectra of six of the major products are anthracene-like and are similar to the previously-reported spectrum of 7,8,9,10-tetrahydro-3-methylcholanthrene, a result that is consistent with metabolic activation involving diol-epoxide formation in the 7, 8, 9, 10-ring of 3-methylcholanthrene. The fluorescence spectra of the other two major products are also anthracene-like, but their maxima are shifted to longer wavelengths relative to the maxima in the spectrum of 7,8, 9,10-tetrahydro-3-methylcholanthrene. The minor product possesses a fluorescence spectrum that is not anthracene-like and that is dissimilar from the spectra of products formed from 3-methylcholanthrene 11,12-oxide.

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