NUTRITIONAL REQUIREMENTS FOR THE REPRODUCTION OF COLIPHAGE T2 IN A STRAIN OF ESCHERICHIA COLI

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Abstract
Coliphage T2 does not undergo reproduction in washed and starved E. coli, strain K12, unless yeast extract, nutrient broth, or casein hydrolysate is added to the synthetic medium M9. This growth factor requirement can be satisfied by a mixture of amino acids. An absolute requirement for isoleucine was demonstrated in mixtures of amino acids tested although it did not stimulate phage synthesis when tested alone. Phenylalanine was inhibitory in the mixtures used, and tryptophan was shown to have a stimulatory effect only in conjunction with a hydrochloric acid hydrolysate of casein. Bacteria not subjected to starvation were capable of supporting phage synthesis. Evidence is presented in support of the view that the washing and starvation procedures deprive the cell of some phage growth promoting substances.

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