Cholera Toxin Crystals Suitable for X-ray Diffraction
- 23 September 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 197 (4310) , 1277-1279
- https://doi.org/10.1126/science.197.4310.1277-a
Abstract
Large crystals of the cholera toxin were grown; their dimensions, symmetry (P21), order, and resistance to radiation make them ideally suited for a high-resolution x-ray structure determination. There is one molecule (approximately 84,000 daltons) per asymmetric unit, and therefore the lattice reveals no molecular symmetry. Two distinct bioassays indicate that the protein from dissolved crystals retains full biological activity.This publication has 2 references indexed in Scilit:
- Crystallization of proteins from polyethylene glycol.Journal of Biological Chemistry, 1976
- Intestinal Secretion: Effect of Cyclic Amp and Its Role in CholeraNew England Journal of Medicine, 1971