DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
- 1 March 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (3) , 1373-1376
- https://doi.org/10.1073/pnas.76.3.1373
Abstract
The feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase (aprt; AMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.7) to the aprt+ phenotype by DNA-mediated gene transfer was demonstrated. Transformation was effected by using unfractionated high MW genomic DNA from Chinese hamster, human and mouse cells and restriction endonuclease-digested DNA from rabbit liver. The transformation frequency observed was 1-10 colonies/106 cells per 20 .mu.g of donor DNA. Transformants displayed enzymatic activity that was donor derived as demonstrated by isoelectric focusing of cytoplasmic extracts. These transformants fall into 2 classes: those that are phenotypically stable when grown in the absence of selective pressure and those that are phenotypically unstable under the same conditions.This publication has 14 references indexed in Scilit:
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