Novel Tetracycline Resistance Gene, tet (32), in the Clostridium -Related Human Colonic Anaerobe K10 and Its Transmission In Vitro to the Rumen Anaerobe Butyrivibrio fibrisolvens

Abstract
A novel tetracycline resistance gene, designated tet (32), which confers a high level of tetracycline resistance, was identified in the Clostridium- related human colonic anaerobe K10, which also carries tet (W). tet (32) was transmissible in vitro to the rumen anaerobe Butyrivibrio fibrisolvens 2221 R . The predicted gene product of tet (32) has 76% amino acid identity with Tet(O). PCR amplification indicated that tet (32) is widely distributed in the ovine rumen and in porcine feces.

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