The PROS-35 gene encodes the 35 kd protein subunit of Drosophila melanogaster proteasome.

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Abstract
The proteasome is a multicatalytic proteinase complex composed of nonidentical protein subunits. We have isolated a cDNA clone encoding the 35 kd proteasome subunit of Drosophila melanogaster and propose the designation PROS‐35 for the corresponding gene. The deduced amino acid sequence reveals a region of striking homology to a tyrosine phosphorylation site of viral and cellular proteins suggesting a potential regulatory function for the 35 kd subunit within the proteinase complex. Immunocytochemical experiments reveal a tissue‐dependent differential distribution of the proteasome between the nucleus and cytoplasm. In addition developmental analysis shows that the proteasome is highly expressed in the CNS of stage‐16 embryos and in cardia, ventriculus and ovaries of adult flies. These data suggest a tissue‐ and development‐dependent distribution of the proteasome in D. melanogaster.