Human Myelin/Oligodendrocyte Glycoprotein: A New Member of the L2/HNK‐1 Family
- 1 November 1993
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 61 (5) , 1822-1827
- https://doi.org/10.1111/j.1471-4159.1993.tb09822.x
Abstract
Myelin/oligodendrocyte glycoprotein (MOG) is a quantitatively minor component of CNS myelin. In this study, human MOG was found to express the L2/HNK-1 epitope on N-linked oligosaccharide structures. This carbohydrate epitope has been found previously in three other characterized human myelin glycoproteins: the myelin-associated glycoprotein, P0, and the oligodendrocyte-myelin glycoprotein. It seems, therefore, that the L2/HNK-1 epitope is expressed frequently in human myelin glycoproteins. Serial lectin affinity chromatography of 14C-glycopeptides indicated that MOG N-oligosaccharide structures are mainly of the complex type, accounting for 77.8% of total radioactivity. In contrast with myelin-associated glycoprotein and P0, which express the L2/HNK-1 epitope on fucosylated structures, in MOG the epitope was detected on all glycopeptide fractions obtained by serial lectin affinity chromatography, although a preferential expression of the L2/HNK-1 epitope was observed on fucosylated structures. Finally, the data indicated that, as for other human myelin glycoproteins, only a subpopulation of MOG molecules expresses the L2/HNK-1 epitope.Keywords
This publication has 26 references indexed in Scilit:
- Families of Neural Adhesion MoleculesPublished by Wiley ,2007
- Myelin/oligodendrocyte glycoprotein is a unique member of the immunoglobulin superfamilyJournal of Neuroscience Research, 1992
- Comparison of the N‐Linked Oligosaccharide Structures of the Two Major Human Myelin Glycoproteins MAG and P0: Assessment of the Structures Bearing the Epitope for HNK‐1 and Human Monoclonal Immunoglobulin M Found in Demyelinating NeuropathyJournal of Neurochemistry, 1992
- Antibodies to myelin-oligodendrocyte glycoprotein in cerebrospinal fluid from patients with multiple sclerosis and controlsJournal of Neuroimmunology, 1991
- Demyelination Induced in Aggregating Brain Cell Cultures by a Monoclonal Antibody Against Myelin/Oligodendrocyte GlycoproteinJournal of Neurochemistry, 1990
- The Epitope(s) Recognized by HNK‐1 Antibody and IgM Paraprotein in Neuropathy Is Present on Several N‐Linked Oligosaccharide Structures on Human P0 and Myelin‐Associated GlycoproteinJournal of Neurochemistry, 1990
- The oligodendrocyte-myelin glycoprotein belongs to a distinct family of proteins and contains the HNK-1 carbohydrate.The Journal of cell biology, 1990
- A phosphatidylinositol-linked peanut agglutinin-binding glycoprotein in central nervous system myelin and on oligodendrocytes.The Journal of cell biology, 1988
- Occurrence of the HNK-1 epitope (3-sulfoglucuronic acid) in PC12 pheochromocytoma cells, chromaffin granule membranes, and chondroitin sulfate proteoglycansBiochemical and Biophysical Research Communications, 1987
- STUDIES ON AUTOIMMUNE ENCEPHALOMYELITIS IN THE GUINEA PIG—TIT. A COMPARATIVE STUDY OF TWO AUTOANTIGENS OF CENTRAL NERVOUS SYSTEM MYELIN1Journal of Neurochemistry, 1979