Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
Top Cited Papers
- 9 August 2006
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 3 (10) , 793-796
- https://doi.org/10.1038/nmeth929
Abstract
We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.Keywords
This publication has 15 references indexed in Scilit:
- Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteinsProceedings of the National Academy of Sciences, 2005
- Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolutionProceedings of the National Academy of Sciences, 2005
- Short-Range Spectroscopic Ruler Based on a Single-Molecule Optical SwitchPhysical Review Letters, 2005
- Carbocyanine Dyes as Efficient Reversible Single-Molecule Optical SwitchJournal of the American Chemical Society, 2005
- Nonlinear magic: multiphoton microscopy in the biosciencesNature Biotechnology, 2003
- Toward fluorescence nanoscopyNature Biotechnology, 2003
- Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm LocalizationScience, 2003
- Precise Nanometer Localization Analysis for Individual Fluorescent ProbesPublished by Elsevier ,2002
- 3-Dimensional super-resolution by spectrally selective imagingChemical Physics Letters, 1998
- Tracking kinesin-driven movements with nanometre-scale precisionNature, 1988