Method for Monitoring and Establishing Baseline Data for Resistance to Permethrin by Liriomyza trifolii (Burgess)

Publisher Website
Google Scholar
Abstract
We describe a method for the microapplication of insecticides to small Agromyzidae, specifically Liriomyza trifolii (Burgess) (Diptera: Agromyzidae). Standardizing the age and sex of the test animals significantly reduced variability and control mortality. Males were more variable in their response to insecticides than females. Short exposures to carbon dioxide for anesthesia did not cause significant mortality; control mortality was typically under 10%. A procedure for increasing susceptibility of a strain of L. trifolii collected in the field is described using 25 isofemale lines. Six susceptible lines that had a range of response to the parent colony LD50 of 95 to 100% mortality were used to initiate a colony. After three generations, this colony has retained this level of sensitivity to permethrin.