STRUCTURAL-ANALYSIS OF CGMP-DEPENDENT PROTEIN-KINASE USING LIMITED PROTEOLYSIS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 255  (15) , 7067-7070
Abstract
CGMP-dependent protein kinase from bovine lung is labile to specific proteolysis. Limited digestion with chymotrypsin produces a 65,000-dalton monomer and a 16,000-dalton dimer from a 150,000-dalton dimeric enzyme. The larger proteolytic fragment represents the COOH-terminal portion of the enzyme and contains the catalytic site along with the cGMP binding site. The smaller fragment representing the NH2-terminal portion of the enzyme contains the autophosphorylation site and the interchain disulfide bond(s). A model defining the functional domains of cGMP-dependent protein kinase is presented and comparisons with cAMP-dependent protein kinase regulatory subunit are discussed.

This publication has 1 reference indexed in Scilit: