Variables that may influence the alkaline sedimentation pattern of herpes simplex virus DNA
- 1 September 1981
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 68 (3-4) , 221-228
- https://doi.org/10.1007/bf01314575
Abstract
Herpes simplex virus (HSV) DNA sediments homogeneously on a neutral sucrose gradient but heterogeneously on an alkaline sucrose gradient. Several factors that may influence the alkaline sedimentation pattern of HSV DNA were examined: e.g., the host cell, cell density at time of infection, multiplicity of infection, and the starting material for HSV DNA purification (whether HSV-infected cells or cell-free virus). Based on alkaline sedimentation analysis, these factors appear to play little or no role in the amount of intact single-stranded HSV DNA observed.Keywords
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