Recombination During Gene Transfer into Mouse Cells Can Restore the Function of Deleted Genes
- 14 January 1983
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 219 (4581) , 174-176
- https://doi.org/10.1126/science.6294829
Abstract
Two plasmids containing nonoverlapping deletions of the herpes simplex virus thymidine kinase gene were introduced into thymidine kinase-deficient mouse L cells by DNA-mediated gene transfer. Thymidine kinase-producing transformants were generated by a mixture of the two plasmids at a frequency significantly greater than that generated by either plasmid alone. Southern blot analyses demonstrated that functional thymidine kinase genes were generated by homologous recombination between the two deletion mutants.Keywords
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