Use of proteolytic enzymes to facilitate the recovery of chitin from shrimp wastes
- 1 November 1993
- journal article
- research article
- Published by Taylor & Francis in Food Biotechnology
- Vol. 7 (3) , 253-263
- https://doi.org/10.1080/08905439309549861
Abstract
Two proteolytic enzymes, chymotrypsin and papain, were used to hydrolyze proteins associated with demineralized shrimp waste to recover chitin. The conditions used were optimized using response surface methodology (RSM). The factors studied were temperature (3 levels), pH (3 levels) and enzyme to waste ratio (E/W ratio)(5 levels). Of these, pH was the most important factor in the models obtained. Optimum conditions for deproteinization by chymotrypsin were determined to be around 40°C, pH 8.0 and E/W ratio of 7.1000 (w:w). With papain, a temperature of around 38°C, pH of 8.7 and E/W ratio of 10:1000 (w:w) gave the optimum response. At these conditions, the yield of protein was maximum, and ranged from 22–48% depending on the starting material. The residual protein levels in the waste after deproteinization with the enzymes were very low, i.e., 1.3 and 2.8% for chymotrypsin and papain‐treated samples, respectively.Keywords
This publication has 2 references indexed in Scilit:
- Chitin variability with species and method of preparationComparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1981
- Determination of protein: A modification of the lowry method that gives a linear photometric responseAnalytical Biochemistry, 1972