Glucose-6-Phosphate Dehydrogenase Overexpression Decreases Endothelial Cell Oxidant Stress and Increases Bioavailable Nitric Oxide
- 1 March 2003
- journal article
- Published by Wolters Kluwer Health in Arteriosclerosis, Thrombosis, and Vascular Biology
- Vol. 23 (3) , 411-417
- https://doi.org/10.1161/01.atv.0000056744.26901.ba
Abstract
Objective— Glucose-6-phosphate dehydrogenase (G6PD), the principal source of NADPH, serves as an antioxidant enzyme to modulate the redox milieu and nitric oxide synthase activity. Deficient G6PD activity is associated with increased endothelial cell oxidant stress and diminished bioavailable nitric oxide (NO·). Therefore, we examined whether overexpression of G6PD would decrease reactive oxygen species accumulation and increase bioavailable NO· in endothelial cells.Keywords
This publication has 20 references indexed in Scilit:
- Expression of 5-lipoxygenase in pulmonary artery endothelial cellsBiochemical Journal, 2002
- Crystal Structure of the FAD/NADPH-binding Domain of Rat Neuronal Nitric-oxide SynthaseJournal of Biological Chemistry, 2001
- Glucose‐6‐phosphate dehydrogenase deficiency promotes endothelial oxidant stress and decreases endothelial nitric oxide bioavailabilityThe FASEB Journal, 2001
- Cyclic strain modulates resistance to oxidant stress by increasing G6PDH expression in smooth muscle cellsAmerican Journal of Physiology-Heart and Circulatory Physiology, 2000
- A Method for Determination of Pyridine Nucleotides Using a Single ExtractAnalytical Biochemistry, 2000
- High Density Lipoprotein Prevents Oxidized Low Density Lipoprotein-induced Inhibition of Endothelial Nitric-oxide Synthase Localization and Activation in CaveolaeJournal of Biological Chemistry, 2000
- Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader11Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee by the United States Department of Agriculture and does not imply its approval to the exclusion of other products that may be suitable.Free Radical Biology & Medicine, 1999
- Superoxide generation by endothelial nitric oxide synthase: The influence of cofactorsProceedings of the National Academy of Sciences, 1998
- Epiandrosterone and dehydroepiandrosterone affect glucose oxidation and interleukin-1 beta effects in pancreatic islets.Endocrinology, 1996
- Regulation of the activity of glucose-6-phosphate dehydrogenase by NADP+ and NADPHBiochimica et Biophysica Acta (BBA) - Enzymology, 1967