Optimizing Annealing Temperature Overcomes Bias in Bisulfite PCR Methylation Analysis
Open Access
- 1 January 2007
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 42 (1) , 48-58
- https://doi.org/10.2144/000112312
Abstract
Reducing PCR Bias in Methylation AnalysisMany analyses of DNA methylation commence with bisulfite treatment of genomic DNA, which converts unmethylated cytosines to uracils, leaving methylated cytosines unchanged....Keywords
Funding Information
- leukemia Specialized Program of Research Excellence (SPORE) Career Development Award (5 P501 Ca100632 03)
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