Proton NMR and NOE Structural and Dynamic Studies of Larger Proteins and Nucleic Acids Aided by Isotope Labels: T4 Lysozyme
- 1 August 1987
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 5 (1) , 21-34
- https://doi.org/10.1080/07391102.1987.10506372
Abstract
This article reviews methods based on direct observation of proton NMR in macromolecules containing 13C or 15N labels. The resonances and Overhauser effects of protons attached to the labels can be edited or filtered from the remaining overlapping resonances. This leads to simplification of the spectra when labels are incorporated selectively. In 2D and related methods the label's chemical shift provides a second dimension which is useful for spectral differentiation and identification. The methods are useful for larger proteins and we describe our progress on studies of T4 lysozyme, mass 18.7 kD, in which we have already identified a large number of resonances.This publication has 13 references indexed in Scilit:
- Proton NMR measurements of bacteriophage T4 lysozyme aided by 15N isotopic labeling: structural and dynamic studies of larger proteins.Proceedings of the National Academy of Sciences, 1987
- Assignment of proton amide resonances of T4 lysozyme by carbon-13 and nitrogen-15 multiple isotopic labelingJournal of the American Chemical Society, 1986
- Two-dimensional proton NMR studies of cytochrome c: assignment of the N-terminal helixBiochemistry, 1986
- Isotope-detected 1H NMR studies of proteins: a general strategy for editing interproton nuclear Overhauser effects by heteronuclear decoupling, with application to phage lambda repressor.Proceedings of the National Academy of Sciences, 1986
- Proton-nitrogen-15 heteronuclear NMR studies of Escherichia coli thioredoxin in samples isotopically labeled by residue typeBiochemistry, 1985
- High Resolution NMR Studies of Nucleic Acids and ProteinsAnnual Review of Physical Chemistry, 1985
- Nuclear magnetic resonance observation and dynamics of specific amide protons in T4 lysozymeBiochemistry, 1985
- Nitrogen-15-labeled yeast tRNAPhe: double and two-dimensional heteronuclear NMR of guanosine and uracil ring NH groupsBiochemistry, 1984
- Correlation of proton and nitrogen-15 chemical shifts by multiple quantum NMRJournal of Magnetic Resonance (1969), 1983
- Pulsed Nuclear Resonance SpectroscopyPhysical Review B, 1960