Determination of Plasma Cortisol by a Fluorometric Method
- 1 November 1964
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 24 (11) , 1188-1194
- https://doi.org/10.1210/jcem-24-11-1188
Abstract
The method of Van der Vies (5) for the determination of plasma cortisol has been investigated and found to be a reliable method for routine clinical purposes. This method does not involve chromatography, and a trained technician can obtain a result within 5 hr, taking the fluorescence reading 10 min after adding the reagent and omitting the preliminary light petroleum washing of the plasma. Under these conditions the normal plasma cortisol level at 8 am is 14.6 ± 3.7 μg/100 ml.Keywords
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