Gangliosides of Calf Thymus and of Normal and Leukemic Bovine Lymphocytes

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Abstract
The gangliosides of calf thymus and of lymphocytes from blood, lymph nodes, lymph and spleen of normal and leukemic cows were investigated in an attempt to determine whether there exists a relation between the ganglioside composition and the maturity of the lymphocytes. With all normal peripheral lymphocytes studied the largely prevailing (up to 97%) ganglioside component was N-glycoloylneuraminosyllactosylceramide. The ganglioside spectrum of calf thymus was much more complex and included at least 6 different components. By TLC, methylation analysis and neuraminidase treatment they were identified as N-glycoloylneuraminosyl and N-acetylneuraminosyl lactosylceramides, N-glycoloyneuraminosylneolactotetraosylceramide, di-(N-glycoloylneuraminosyl), di-(N-acetylneuraminosyl) lactosylceramides and N-glycololyneuraminosyl-N-acetylneuraminosyllactosylceramide. The ganglioside spectra of leukemic peripheral lymphocytes differed from those of normal peripheral lymphocytes and resembled the ganglioside profile of the thymus. In the normal animal, the development of thymocytes into mature peripheral lymphocytes is accompanied by loss of disialosylgangliosides and sialosylneolactotetraosylceramide. Both the structure and the composition of the lymphocyte gangliosides become more simple during maturation and aging of the lymphocyte.

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