Effect of 3':5'-cyclic AMP on glucose transport in rat adipocytes.

Abstract
An indirect method for obtaining a reliable measure of the rate of glucose transport into adipocytes is described. Evidence is presented that altered levels of 3'':5''-cyclic[c]AMP can incluence the transport of glucose into adipocytes. When cAMP levels were lowered with antilipolytic agents (insulin, nicotinic acid, or clofibrate), rates of glucose transport were increased. In contrast, when adipose tissue levels of cAMP were elevated by lipolytic hormones or theophylline, glucose transport was inhibited. Insulin no longer increased glucose transport when cAMP levels were elevated by lipolytic agents. Agents that can raise cAMP but inhibit lipolysis (procaine, amitryptyline, and phenylethylbiguanide) reduced the rate of glucose transport. Other data are presented that are consistent with the conclusion that cAMP inhibits glucose transport into adipocytes.

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