Partial Organogenesis in Tissue Cultures of Averrhoa carambola1

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Abstract
Callus was initiated from seedling leaf explants and from leaves of recent vegetative flushes of mature Averrhoa carambola L. trees on solid Murashige and Skoog medium with added growth regulators. Seedling-derived callus demonstrated a strong dependence on the presence of exogenous cytokinin, 1-5 mg/liter 6(γ, γ-dimethylallylamino) purine (2iP) with 0.2 mg/liter 2,4-dichlorophenoxyacetic acid (2,4-D), for induction and growth. Shoot differentiation was stimulated from seedling callus by increasing the concentration of available cytokinin. Callus induction and growth from young leaves of mature trees was auxin-dependent (2-5 mg/liter 2,4-D with 0.5 mg/liter 2iP. Organogenesis from callus cultures of mature leaf origin was not obtained.

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