Synthesis of type II collagen in vitro by embryonic chick neural retina tissue.

Abstract
Collagen produced by chick neural retina tissue in vitro had the properties of type II collagen. Isolated neural retina tissue from stage 29-32 chick embryos incorporated 14C-glycine and 3H-proline into collagen consisting of .alpha.1 chains and a higher MW species. The peptides produced by CNBr digestion of the collagen were identical to those from authentic type II collagen from cartilage in charge properties, as determined by CM-cellulose chromatography and size distribution, as determined by sodium dodecyl sulfate-acrylamide gel electrophoresis.