Localization of acidic residues involved in the proton pumping activity of the bovine heart mitochondrial bc1 complex
Open Access
- 28 July 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 456 (1) , 37-40
- https://doi.org/10.1016/s0014-5793(99)00920-5
Abstract
Chemical modification of carboxyl residues in polypeptide subunits of the mitochondrial bc1 complex causes a decoupling effect, that is inhibition of the proton pumping activity, without affecting the rate of electron transfer to ferricytochrome c. The study presented here is aimed at localizing and identifying the residues whose modification results in decoupling of the complex. Glutamate‐53 in subunit IX (the DCCD‐binding protein) and aspartate‐166 in the Rieske iron‐sulfur protein are the residues modified by N,N′‐dicyclohexylcarbodiimide (DCCD) and N‐(ethoxycarbonyl)‐2‐ethoxy‐1,2‐dihydroquinoline (EEDQ), respectively. The results obtained also suggest that the carboxy‐terminal sequence of the Core protein II, which is fairly rich in acidic residues, may also play a role in the vectorial proton translocation activity of the complex.Keywords
This publication has 32 references indexed in Scilit:
- Chemical Modification of the Bovine Mitochondrial bc1 Complex Reveals Critical Acidic Residues Involved in the Proton Pumping ActivityBiochemistry, 1998
- Structure of a water soluble fragment of the ‘Rieske’ iron–sulfur protein of the bovine heart mitochondrial cytochrome bc1 complex determined by MAD phasing at 1.5 å resolutionStructure, 1996
- Pathway of Proton Transfer in Bacterial Reaction Centers: Role of Aspartate-L213 in Proton Transfers Associated with Reduction of Quinone to DihydroquinoneBiochemistry, 1994
- Characteristics of energy‐linked proton translocation in liposome reconstituted bovine cytochrome bc1 complexEuropean Journal of Biochemistry, 1992
- Preparation of novel cyclosporin A derivativesBioconjugate Chemistry, 1992
- Small weak acids stimulate proton transfer events in site‐directed mutants of the two ionizable residues, GluL212 and AspL213, in the QB‐binding site of Rhodobacter sphaeroides reaction centerFEBS Letters, 1991
- Structure and function of the mitochondrial bc1 complexJournal of Molecular Biology, 1989
- Redox‐linked proton translocation in the b–c1 complex from beef‐heart mitochondria reconstituted into phospholipid vesiclesEuropean Journal of Biochemistry, 1983
- A new method of coupling proteins to insoluble polymersBiochemical and Biophysical Research Communications, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970