INHIBITORY EFFECT OF INTERFERON ON DNA AND RNA-SYNTHESIS IN MURINE SPLEEN-CELLS STIMULATED BY LECTINS

  • 1 January 1976
    • journal article
    • research article
    • Vol. C127  (2) , 163-171
Abstract
Spleen cells from mice inoculated with partially purified preparations of interferon (SA [specific activity] 1 .times. 107 IU/mg protein, 0.2 ml i.v./mouse) were stimulated in vitro with phytohemagglutinin (PHA), concanavalin A or [Salmonella typhi] lipopolysaccharide. After 2 days of stimulation, the incorporation of 3H-thymidine into TCA-[trichloroacetic acid]-insoluble radioactivity was inhibited 50-90% when compared with cells from animals inoculated with mock interferon. Maximal inhibition, with optimal doses of lectins was obtained when interferon was inoculated 18 h before. This effect of interferon on DNA synthesis was preceeded by inhibition of the incorporation of 3H-uridine into TCA-insoluble material.

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