ISOLATION AND PARTIAL CHARACTERIZATION OF THE PHOTOCHEMICAL REACTION CENTER OF CHROMATIUM VINOSUM (STRAIN D)
- 1 July 1975
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 22 (1-2) , 37-40
- https://doi.org/10.1111/j.1751-1097.1975.tb06718.x
Abstract
Abstract— –A preparation of the photochemical reaction center of Chromatiwn has been obtained by chromatography of lauryldimethylamineoxide‐solubilized chromatophores on hydroxylapatite and Sepharose columns. The procedure has yielded a reaction center preparation from both carotenoid‐containing and carotenoid‐deficient Chromatium cells. Preliminary analysis of the isolated component indicates that the photochemical reaction center of the Thiorhodaceae is homologous to that of the Athiorhodaceae. In particular, the near infrared absorption spectrum of the Chromatium reaction center preparation shows the same triple‐peaked spectrum observed for reaction center preparations from the Athiorhodaceae. The Chromatium preparation undergoes a rapid light‐induced oxidation and dark reduction of the reaction center. The ratio of the reaction center to the two membrane‐bound cytochromes (cytochrome c552 and c555) is greatly increased over the ratio observed in chromatophores or in other previously isolated, reaction center‐enriched subchromatophore fractions of this organism.This publication has 24 references indexed in Scilit:
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