Recombinant Whole Cells as Catalysts for the Enzymatic Synthesis of Oligosaccharides and Glycopeptides
- 6 June 1994
- journal article
- research article
- Published by Wiley in Angewandte Chemie International Edition in English
- Vol. 33 (12) , 1241-1242
- https://doi.org/10.1002/anie.199412411
Abstract
An easier, faster, cheaper approach to the enzymatic synthesis of oligosaccharides would be to employ whole cells rather than purified enzymes. In this report recombinant E. coli cells mediate the mannosylation of monosaccharides and glycopeptides. It was also proven that the relevant enzyme, mannosyl transferase, does not leak from the periplasmic space of the cells into the medium.Keywords
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