Enhancement of glomerular permeability to anionic ferritin induced by kidney perfusion with collagenase

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Abstract
The role of collagenin ultrafiltration properties of the glomerular basement membrane (GBM) was tested after a single administration of bacterial collagenase, using native ferritin as a tracer which does not pass through the GBM under physiological conditions. Experiments were performed both in situ and with isolated kidneys [rat]. Increased permeability to ferritin occurs 6 h following enzyme perfusion and becomes patent after 30 h, numerous tracer molecules appearing in urinary space, without any readily observable changes either in distribution of fixed negative charges (as revelaed by colloidal iron and polyethyleneimine) or in structural organization of the glomerulus. Selective permeability of the GBM is progressively restored so that ferritin is almost confined to capillary lumen 1 mo. after enzyme injection. Apparently, collagen plays an important part in restricting plasma protein filtration.