INHIBITION OF NATURAL-KILLER-CELL MEDIATED CYTOLYSIS WITH MONOCLONAL-ANTIBODIES TO RESTRICTED AND NONRESTRICTED EPITOPES OF THE LEUKOCYTE COMMON ANTIGEN

  • 1 July 1987
    • journal article
    • research article
    • Vol. 61  (3) , 351-356
Abstract
Three monoclonal antibodies recognizing different epitopes of the leucocyte common molecule, CMRF-11 (against the restricted or B-220 leucocyte common molecule), CMRF-12 and CMRF-26 [each against a different epitope on the non-restricted or T200 leucocyte common (CD45) molecule], were tested for their effects on lymphocyte cytotoxity. The individual monoclonal antibodies inhibited human natural killer cell-mediated cytolysis (NK-CMC) weakly, but a mixture of CMRF-11+12+26 antibodies inhibited cytolysis more consistently and to a greater extent. This mixture did not inhibit cytotoxic T lymphocytes derived from secondary mixed lymphocyte cultures. The CMRF-11+12+26 mix was shown to inhibit a post-conjugate formation stage of lysis at the effector cell level. Inhibition of NK-CMC of a wide range of target cells, including the T-cell lines Jurkat, HSB2 and Molt 4, was demonstrated.

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