EFFECTS OF DINITRATO(1,2-DIAMINOCYCLOHEXANE)PLATINUM (NSC-239851) ON MURINE MYELOMA AND HEMATOPOIETIC PRECURSOR CELLS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (9) , 3185-3188
Abstract
The effects of dinitrato(1,2-diaminocyclohexane)platinum (NSC 239851) on murine myeloma Adj. PC-5 and hemopoietic precursor cells were studied. The median survival time of tumor-bearing mice was significantly affected by i.p. injections of this agent. While 14 mg/kg was a toxic dose, as little as 2 mg/kg prolonged survival for over 100 days. When cells from normal marrow and actively regenerating marrows were exposed to the agent in culture tubes, washed and assayed for surviving hemopoietic precursors, similar sensitivity curves were observed. This result indicates the absence of cell cycle dependency of the agent. When marrow cells were exposed to NSC 239851 at 4.degree. C in culture, almost total abrogation of cytotoxicity was noted. This observation, unlike that from the experiment with the inorganic Pt congener, cis-diamminedichloroplatinum, suggests that the transport of this agent is an active process.