SPECIFIC RECEPTORS FOR GLUCOCORTICOID IN CYTOPLASM OF LIVER OF AH 130 TUMOR-BEARING RATS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (12) , 4382-4386
Abstract
Specific receptors for dexamethasone (11.beta.,17.alpha.,21-trihydroxy-9.alpha.-fluoro-16.alpha.-methyl-1,4-pregnadiene-3,20-dione) in the cytoplasm of the liver from AH 130 (solid type) tumor-bearing rats markedly increased in the advanced stage of tumor growth. The cytoplasmic receptors of the livers of normal and tumor-bearing rats differed in their affinities for dexamethasone, and their apparent Kd for dexamethasone were 4.0 and 2.6 .times. 10-9 M, respectively. The rates of dissociation of dexamethasone-receptor complexes and the heat denaturations of the receptors in the livers of normal and tumor-bearing rats were similar. The glucocorticoid receptors of tumor-bearing rat liver had slightly higher affinities than those of normal liver for all steroids tested. Only a trace amount of receptors for dexamethasone could be detected in the cytoplasm of AH 130 ascites cells.

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