Quantitative Ratio of Primer Pairs and Annealing Temperature Affecting PCR Products in Duplex Amplification
Open Access
- 1 October 1999
- journal article
- research article
- Published by Taylor & Francis in BioTechniques
- Vol. 27 (4) , 762-770
- https://doi.org/10.2144/99274st07
Abstract
The quantity of PCR products that are simultaneously amplified from two different loci in a duplex amplification (DA) are significantly lower for one of the loci, as compared to identical PCR ampli...Keywords
This publication has 2 references indexed in Scilit:
- The impact of the PCR plateau phase on quantitative PCRBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1994
- Analysis of the expression immunoglobulin gene repertoire by screening libraries derived from PCR-amplified cDNANucleic Acids Research, 1991