The identification of propionylcholine as a constituent of ox spleen

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Abstract
The separation of propionylcholine from ox spleen by an improved method involving chromatography on ion-exchange resins is described. The spleen propionylcholine was identified as such by its chromatographic and physiological properties and by its infrared absorption spectrum. Reasons are advanced for believing that the values obtained for the total cencentration of acetylcholine and acetylcholine-like substances in spleen tissue, though lower than those reported by earlier workers, represent the true values obtainable with modern assay techniques.