PROPOSAL OF STANDARDIZED METHODS AND REFERENCE FOR ASSAYING RECOMBINANT HUMAN TUMOR NECROSIS FACTOR
- 1 January 1986
- journal article
- research article
- Published by Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Dis in Japanese Journal of Medical Science and Biology
- Vol. 39 (3) , 105-118
- https://doi.org/10.7883/yoken1952.39.105
Abstract
Two assay methods for recombinant human tumor necrosis factor (rH-TNF) were developed, one a biological L-cell assay and the other an enzyme-linked immunosorbent assay. The accuracy and reproducibility of each and the correlation between the two were studied. As a result of this investigation, the two assay methods were found appropriate for standardization of rH-TNF. A freeze-dried reference was prepared, and examination of its potency and stability showed it to be suitable for use as a reference standard for rH-TNF assays.This publication has 8 references indexed in Scilit:
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